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Trypane Blue Exclusion

You have got 5 ml of a cell suspension. 10 microliter of this suspension is mixed with 10 microliter of the trypane blue solution and placed into a Neubauer Counting chamber. Now its your turn to determine the percentage and the total number of viable cells!

Step 1: Please count the cells!



Your total cell count:              

Your number of viable cells:

Next step!