Contents

Chapter 1
Anatomy of the
immune system

Chapter 2
Important molecules

Chapter 3
The development of
the immune system

Chapter 4
The physiology of
the immune response

Chapter 5
Immunity to infections

Chapter 6
Pathological reactions
of the immune system

Chapter 7
The immune defence against tumours

Chapter 8
Transplantations

Chapter 9
Immunological methods

9.1. Antibodies as tools
        - Flow cytometry (FACS)
        - Immune scintigraphy
        - Recombinant antibodies

9.2. Detection of antigens and antibodies
        - ELISA        
        - Immune electrophoresis

9.3. Isolation and functional characterization of cells
        - Counting chamber   
        - Counting antigen-specific T cells using Tetrameric peptide-MHC-complexes    
        - Measuring cell proliferation
                - CFSE
        - Cell death
                - Determination of cell viability    
                - DNA ladder
                - TUNEL
        - Analysis of activation-induced gene expression
                - Subtraction cDNA-library

9.4. Investigating the immune system in vivo
        - Cancer vaccination
        - The Cre-lox system
        - DNA vaccination