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Subtraction cDNA-library | |
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Introduction Subtractive cDNA cloning is a powerful technique which allows the selective amplification of genes which are differentially transcribed, for example in activated but not in resting cells or in one tissue but not in another. With the help of substrated cDNA libraries these genes can be isolated and identified. After working through this topic you should be able to explain the construction of a PCR-based substracted cDNA library. The method The construction of a subtracted cDNA library is described in the milestone „AID, an enzyme essential for class switching and somatic hypermutation“.  Related topic  AID, an enzyme essential for class switching and somatic hypermutation |
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