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Tetrameric peptide-MHC complexes | |
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Introduction Limiting dilution assays may underestimate the number of specific T cells because this technique cannot detect cells with low or none proliferative potential. Yet, staining specific T cells with their physiological ligand fails because soluble peptide-MHC-complexes dissociate very rapidly form the T cell receptor. After working through this topic you should be able to explain - how MHC molecules can be linked together - why multimeric MHC complexes are advantageous for staining T cells of a given specificity The method  Related paper Altman JD, Moss PA, Goulder PJ, Barouch DH, McHeyzer-Williams MG, Bell JI, McMichael AJ, Davis MM. 1996. Phenotypic analysis of antigen-specific T lymphocytes. Science. Oct 4;274(5284):94-6. |
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