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Apoptosis TUNEL reaction | |
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Introduction With increased understanding of the physiological events that occur during apoptosis, a number of assay methods have been developed for its detection. These assays are based on different events which characterize the apoptotic process. Apoptosis and cell mediated cytotoxicity are characterized by a fragmentation of the genomic DNA. The many breakpoints can be visualized with the TUNEL reaction (TdT-dependent dUTP-biotin nick end labelling). The enzyme TdT (terminal desoxynucleotidyl transferase) adds biotinylated nucleotides to the broken DNA ends. The biotinnylated DNA can then be visualized by different methods. The method  Related topic  Apoptosis DNA ladder |
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